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Exploring The Genetics Of Taste: SNP Analysis Of The PTC Gene Using PCR
About This Item
Description
The objective of this experiment is to identify the presence of the single nucleotide polymorphism (SNP) in an amplified segment of the PTC gene that links detection of the characteristic taste of PTC paper. This is a set of five modules that starts with (I) extraction of DNA from buccal cells (II) amplifying the segment that contains the polymorphic nucleotide (III) digestion of the amplified fragment with the restriction enzyme that recognizes the SNP (IV) analysis by gel electrophoresis (V) tasting the PTC paper to confirm the results obtained.Features
- Set of five modules
- Additional resources and guides available on manufacturer's site
- Appropriate for most middle and high school students
Includes:
- Instructions
- PCR EdvoBeads
- Universal DNA Buffer
- TE Buffer
- LyphoPrimer Mix
- LyphoControl (complete PCR control)
- Dryzymes Restriction Enzyme HaeIII
- Restriction Enzyme Dilution Buffer
- 100 bp Ladder
- Proteinase K
- PTC Paper
- Control Taste Paper
- UltraSpec-Agarose
- TBE Electrophoresis Powder Buffer
- SYBR Safe Stain
- Disposable Plastic Cups
- 15 mL Conical Tube
- Snap-top Microcentrifuge Tubes
- 1.5 ml Screw-cap Microcentrifuge Tubes
- 0.2 ml PCR Tubes
- Salt Packets
Specifications
Allergens:
Contains No Allergens
Maximum Age:
99
Maximum Grade Level:
No Max
