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Edvotek Investigating Synthetic Biology
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Description
Teach your students about synthetic biology with this exciting and exclusive lab! Students use PCR to amplify the coding sequence of the BSMT1 enzyme. This interesting enzyme is responsible for the formation of methyl salicylate, a In this lab activity, students will investigate synthetic biology using the PCR to discover the coding sequence of the BSTM1 enzyme. The PCR product provides a resulting recombinant DNA that is used to transform E. coli BactoBeads, allowing students to perform a smell test experiment ensuring the bacterial factories are working from the enzyme.- PCR EdvoBeads
- Primer mix concentrate
- Template DNA concentrate
- Universal DNA buffer
- Ultrapure water
- DNA standard marker
- 10X gel loading solution
- Purification and wash buffers
- Spin columns and reservoirs
- Dryzyme mix
- Dryzyme reconstitution buffer
- Enzyme reaction buffer
- vector cut with EcoRI and HindIII
- T4 DNA ligase
- ReadyPour LB agar
- LB medium
- BactoBeads (Host)
- Calcium Chloride
- Competent cell solution
- Control DNA
- Recovery broth
- Kanamycin
- IPTG
- Salacylic acid
- wintergreen scent
- 50X electrophoresis buffer
- UltraSpec-Agarose
- SYBR Safe Stain
- microcentrifuge and PCR tubes
- 10 ml pipet
- 50 ml conical tubes
- Sterile loops.
Specifications
Safety:
Allergens:
Contains No Allergens
Certifications:
Not Applicable
No Choking Hazard